Coding

Part:BBa_K590015:Design

Designed by: Michael Brasino, Alicia Wong, Rashmi Ravichandran   Group: iGEM11_Washington   (2011-09-15)


sfGFP_mamK_pGA1C3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1430
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1430
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1134
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1430
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1430
    Illegal NgoMIV site found at 1156
    Illegal NgoMIV site found at 1523
    Illegal NgoMIV site found at 1670
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 80
    Illegal SapI.rc site found at 1802


Design Notes

sequence confirmed


Source

Made from Gibson reaction (Miniprepped)

References